3GVO
Structure and RNA binding of the mouse Pumilio-2 Puf Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.05 |
Spacegroup name | H 3 2 |
Unit cell lengths | 142.393, 142.393, 112.233 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.720 - 1.600 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ib2 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.347 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.045 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.067 | 0.393 |
Total number of observations | 15337 | |
Number of reflections | 56410 | |
<I/σ(I)> | 15 | 1.7 |
Completeness [%] | 98.3 | 92.3 |
Redundancy | 5.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | 0.2M ammonium citrate, 20%(w/v) PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |