3GVD
Crystal Structure of Serine Acetyltransferase CysE from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97937 |
Spacegroup name | P 1 |
Unit cell lengths | 68.472, 81.228, 139.406 |
Unit cell angles | 97.07, 95.32, 94.72 |
Refinement procedure
Resolution | 45.986 - 2.400 |
R-factor | 0.2022 |
Rwork | 0.199 |
R-free | 0.26490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t3d |
RMSD bond length | 0.008 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.170 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.095 | 0.383 |
Number of reflections | 112894 | |
<I/σ(I)> | 6.1 | 2.2 |
Completeness [%] | 97.5 | 96.7 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Potassium acetate, 20% w/v Polyethylene glycol 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |