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3GUE

Crystal Structure of UDP-glucose phosphorylase from Trypanosoma Brucei, (Tb10.389.0330)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU FR-E+ SUPERBRIGHT
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2009-03-13
DetectorRIGAKU RAXIS IV++
Wavelength(s)1.5418
Spacegroup nameC 1 2 1
Unit cell lengths167.697, 77.485, 112.209
Unit cell angles90.00, 117.88, 90.00
Refinement procedure
Resolution25.000 - 1.920
R-factor0.243
Rwork0.241
R-free0.29400
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2oeg
RMSD bond length0.016
RMSD bond angle1.519
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareBALBES
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.990
High resolution limit [Å]1.9201.920
Rmerge0.1210.707
Number of reflections96096
<I/σ(I)>10.4521.33
Completeness [%]98.790.6
Redundancy3.13
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.529322% PEG3350, 0.1M Ammonium sulfate, 0.1M Bis Tris, 18% glycerol, 2 mM UDP-glucose, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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