3GTV
Human-mouse SOD1 chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-12 |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.625, 144.007, 112.613 |
| Unit cell angles | 90.00, 119.82, 90.00 |
Refinement procedure
| Resolution | 40.350 - 2.200 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vr7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.198 | 2.200 |
| Number of reflections | 148445 | |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 94.0 | 90 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 0.1 m HEPES, 25% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
