3GPL
Crystal structure of the ternary complex of RecD2 with DNA and ADPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-20 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.120, 130.360, 80.050 |
Unit cell angles | 90.00, 107.34, 90.00 |
Refinement procedure
Resolution | 43.270 - 2.500 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3e1s |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.047 | 0.251 |
Number of reflections | 44090 | |
<I/σ(I)> | 16.05 | 3.83 |
Completeness [%] | 98.4 | 94.6 |
Redundancy | 2.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 10% PEG 8000, 100 mM Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | Tris-HCl | ||
3 | 1 | 2 | PEG 8000 | ||
4 | 1 | 2 | Tris-HCl |