3GOD
Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated antiviral defense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97856,1.884244 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.870, 110.960, 130.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.974 - 2.170 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.643 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.974 | 2.230 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 162078 | |
| <I/σ(I)> | 7.96 | 2.01 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 250 mM CaCl2, 50 mM HEPES pH 7.6, 10% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
