3GO5

Crystal structure of a multidomain protein with nucleic acid binding domains (sp_0946) from streptococcus pneumoniae tigr4 at 1.40 A resolution

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97839
Spacegroup name	P 32 2 1
Unit cell lengths	62.560, 62.560, 160.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.136 - 1.400
R-factor	0.155
Rwork	0.154
R-free	0.17500
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.420
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.136	29.136	1.450
High resolution limit [Å]	1.400	3.010	1.400
Rmerge	0.085	0.042	0.979
Number of reflections	72475	13898	13414
<I/σ(I)>		33.2	1.7
Completeness [%]	99.6	99.8	96.7
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	NANODROP, 0.3640M Ammonium dihydrogen phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K