3GNX
Structure of dehydrated D-xylose isomerase from streptomyces rubiginosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2007-10-10 |
Detector | STOE |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.853, 87.650, 94.531 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.350 - 2.000 |
R-factor | 0.16254 |
Rwork | 0.160 |
R-free | 0.20640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xig |
RMSD bond length | 0.016 |
RMSD bond angle | 1.615 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 94.490 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.137 |
Number of reflections | 51870 |
<I/σ(I)> | 8.1 |
Completeness [%] | 98.3 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 278 | 6mM Tris-HCl, 33mg/ml crystalline suspension, 0.91M ammonium sulfate, 1mM magnesium sulfate (Hampton Research Ltd.); Soaked in xylitol solution, pH 7.0, temperature 278K |