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3GLY

REFINED CRYSTAL STRUCTURES OF GLUCOAMYLASE FROM ASPERGILLUS AWAMORI VAR. X100

Replaces:  1GLY
Experimental procedure
Spacegroup nameP 21 21 21
Unit cell lengths116.700, 104.300, 48.490
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.000 - 2.200
R-factor0.141
RMSD bond length0.014
RMSD bond angle0.029
Refinement softwarePROLSQ
Data quality characteristics
 Overall
High resolution limit [Å]2.100

*

Rmerge0.044

*

Total number of observations233315

*

Number of reflections33871

*

Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

5.95

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein15 (mg/ml)
21droppotassium phosphate50 (mM)
31dropPEG600013 (%(w/v))
41reservoirphosphate50 (mM)
51reservoirPEG600030 (%(w/v))

227111

PDB entries from 2024-11-06

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