3GLR
Crystal Structure of human SIRT3 with acetyl-lysine AceCS2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-25 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 78.190, 129.063, 77.899 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.000 - 1.800 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.22600 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.443 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNX |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.412 |
Number of reflections | 36839 | |
<I/σ(I)> | 17.7 | 3.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 291 | 0.2 M lithium sulfate monohydrate, 15% (w/v) PEG 12000 and 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |