3GLK

The biotin carboxylase (BC) domain of human Acetyl-CoA Carboxylase 2 (ACC2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-06-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 43 2 2
Unit cell lengths	97.536, 97.536, 127.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.280 - 2.100
R-factor	0.212
Rwork	0.212
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.300	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.100	0.673
Number of reflections	35837
<I/σ(I)>	28.6	4.2
Redundancy	8.9	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M TRIS, 0.1M MG FORMATE, 20% PEG 4K, 15% GLYCEROL, 5 MM DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K