3GLC
Crystal Structure of E. coli LsrF in complex with Ribose-5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.352, 105.451, 173.415 |
| Unit cell angles | 89.51, 79.79, 90.34 |
Refinement procedure
| Resolution | 55.730 - 2.500 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gkf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.358 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.6) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.730 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.065 | 0.279 |
| Number of reflections | 154484 | |
| <I/σ(I)> | 7.5 | 1.8 |
| Completeness [%] | 81.7 | 67.8 |
| Redundancy | 2.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 4% PEG 400, 100 mM MgCl2, 2.3 M Ammonium Sulfate, pH 7.5, vapor diffusion, temperature 298K |
