3GKW
Crystal structure of the Fab fragment of Nimotuzumab. An anti-epidermal growth factor receptor antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.223, 84.223, 138.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.790 - 2.500 |
| R-factor | 0.21747 |
| Rwork | 0.214 |
| R-free | 0.28375 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uyw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.701 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.125 | 0.489 |
| Number of reflections | 17486 | |
| <I/σ(I)> | 16.7 | 3.6 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 293 | 0.1 M MES, 25% PEG 1000, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
