3GJ9
crystal structure of TIP-1 in complex with c-terminal of Kir2.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2008-10-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 86.457, 86.457, 101.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.270 - 2.800 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3diw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 10830 | |
<I/σ(I)> | 29.6 | 2.9 |
Completeness [%] | 97.7 | 81.1 |
Redundancy | 8.9 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG8000, cacodylate buffer, zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |