3GJ8
Crystal structure of human RanGDP-Nup153ZnF34 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.257, 61.713, 70.635 |
| Unit cell angles | 90.00, 112.31, 90.00 |
Refinement procedure
| Resolution | 33.754 - 1.820 |
| R-factor | 0.1718 |
| Rwork | 0.170 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entru 3GJ3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.943 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 52824 | |
| <I/σ(I)> | 19.3 | 1.97 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M Bis-Tris pH 6.5, 18-20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
