3GG5
Replacement of Val3 in Human Thymidylate Synthase Affects Its Kinetic Properties and Intracellular Stability
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Detector technology | CCD |
Collection date | 2008-10-04 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.562, 94.864, 130.864 |
Unit cell angles | 90.00, 122.43, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.770 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.25400 |
RMSD bond length | 0.053 |
RMSD bond angle | 4.453 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.086 | 0.537 |
Number of reflections | 36191 | |
<I/σ(I)> | 10.572 | |
Completeness [%] | 88.2 | 67 |
Redundancy | 2.8 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris,PH8.0, 30mM ammonium sulfate, 24%PEG4000, 20mM BME , VAPOR DIFFUSION, HANGING DROP, temperature 277K |