3GEQ
Structural basis for the chemical rescue of Src kinase activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.649, 63.462, 83.860 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
Rwork | 0.226 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2src |
RMSD bond length | 0.074 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 31912 | |
Completeness [%] | 98.7 | 93.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 50 mM Ammoniumacetate, 100 mM Bis-Tris pH 5.5, 1 mM DTT, 50 mM imidazole, 0.5 mM PP2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |