3GE1
2.7 Angstrom Crystal Structure of Glycerol Kinase (glpK) from Staphylococcus aureus in Complex with ADP and Glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.412, 193.651, 91.844 |
| Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.116 | 0.583 |
| Number of reflections | 56438 | |
| <I/σ(I)> | 18.9 | 2.6 |
| Completeness [%] | 97.2 | 83.1 |
| Redundancy | 7.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | 2% Glycerol, PEG3350 20%, 0.2M Sodium dihydrogen Phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
