3GD8
Crystal Structure of Human Aquaporin 4 at 1.8 and its Mechanism of Conductance
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.11 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 82.058, 82.058, 76.353 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.130 - 1.800 |
R-factor | 0.1599 |
Rwork | 0.160 |
R-free | 0.16453 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.047 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.600 |
Number of reflections | 23583 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 25% PEG-2K-MME, 50mM Citrate, pH 6.0, 40mM OG, 5% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |