3GD4
Crystal structure of the reduced, NAD-bound form of murine apoptosis inducing factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2002-10-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2398 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.260, 120.390, 178.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.670 - 2.240 |
| R-factor | 0.18861 |
| Rwork | 0.187 |
| R-free | 0.22209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gv4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.671 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.670 | 2.300 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.042 | 0.333 |
| Number of reflections | 57099 | |
| <I/σ(I)> | 3.57 | |
| Completeness [%] | 95.1 | 85.3 |
| Redundancy | 6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 4000, 10% isopropanol, 0.1 M Li citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
