3GD1
Structure of an Arrestin/Clathrin complex reveals a novel clathrin binding domain that modulates receptor trafficking
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9833, 0.9795, 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 228.960, 61.198, 159.840 |
| Unit cell angles | 90.00, 119.32, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.650 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.069 | 0.236 |
| Number of reflections | 25549 | |
| <I/σ(I)> | 20.2 | 6.3 |
| Completeness [%] | 99.1 | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 293 | 100mM Bicine, 7% O-(2-aminopropyl)-O'-(2-methoxyethyl)polypropylene glycol 500, 6% polyethylene glycol 8000, 4% acetone, 1% ethylene glycol and 10 mM strontium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
