3GCM
Crystal Structure of E. coli polynucleotide phosphorylase bound to RNA and RNase E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2006-10-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 176.335, 176.335, 189.628 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.16911 |
Rwork | 0.166 |
R-free | 0.22065 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e3h |
RMSD bond length | 0.022 |
RMSD bond angle | 1.939 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.619 | |
Number of reflections | 97645 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 99.3 | 99.5 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293.15 | 0.2 M ammonium hydrogen citrate, 17 % w/v PEG 3350, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |