3GBO
Crystal structure of BmooMPalpha-I, a non-hemorrhagic metalloproteinase isolated from Bothrops moojeni snake venom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.428 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.074, 48.732, 54.393 |
| Unit cell angles | 90.00, 91.56, 90.00 |
Refinement procedure
| Resolution | 20.800 - 1.770 |
| R-factor | 0.17412 |
| Rwork | 0.172 |
| R-free | 0.21820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.898 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.800 | 1.830 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 19308 | |
| Completeness [%] | 94.2 | 66.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | PEG 8000, Tris buffer, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
