3GBN
Crystal Structure of Fab CR6261 in Complex with the 1918 H1N1 influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 75 |
| Detector technology | CCD |
| Collection date | 2008-10-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03333 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 201.918, 202.249, 202.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.720 - 2.200 |
| R-factor | 0.20674 |
| Rwork | 0.205 |
| R-free | 0.24142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1ADQ 1ruz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.685 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 71438 | |
| <I/σ(I)> | 30.9 | 2.32 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 295 | 10.5% PEG 3K, 100mM NaAco 5.2, 50mM NaCl, Cryoprotectant: 25% glycerol, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
