3GBM
Crystal Structure of Fab CR6261 in Complex with a H5N1 influenza virus hemagglutinin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 75 |
| Detector technology | CCD |
| Collection date | 2008-10-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0333 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 202.761, 202.761, 202.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.700 |
| R-factor | 0.20586 |
| Rwork | 0.203 |
| R-free | 0.26067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ADQ 2FK0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.432 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 76118 | |
| <I/σ(I)> | 18.4 | 2.03 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 21.4 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 12% PEG 6000, 10mM sodium acetate pH 6.0 Cryoprotectant: 25% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
