3G8A
T. thermophilus 16S rRNA G527 methyltransferase in complex with AdoHcy in space group P61
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-16 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 61 |
| Unit cell lengths | 134.849, 134.849, 167.139 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.140 - 2.100 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.22900 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.099 | 0.489 |
| Number of reflections | 98926 | |
| <I/σ(I)> | 3.14 | |
| Completeness [%] | 98.9 | 99.8 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | containing 20% (w/v) PEG1000, 100 mM imidazole (pH 8.0) and 200 mM calcium acetate, microbatch technique under oil, temperature 277K |
