3G6W
Asymetric GTP bound structure of UPRTase from Sulfolobus solfataricus containing PRPP-mg2+ in half of the active sites and R5P and PPi in the other half
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2004-11-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.954 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.038, 98.758, 73.528 |
Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.900 |
Rwork | 0.216 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xtt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.098 | 0.397 |
Number of reflections | 19229 | |
<I/σ(I)> | 14 | 3.4 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% PEG8000, 0.1M sodium cacodylate, pH6.5, 200mM magnesium chloride, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |