3G6O
Crystal structure of P. aeruginosa bacteriophytochrome PaBphP photosensory core domain mutant Q188L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-21 |
Detector | MAR CCD 165 MM 315 |
Spacegroup name | P 65 |
Unit cell lengths | 109.786, 109.786, 189.113 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.470 - 2.850 |
R-factor | 0.232 |
Rwork | 0.229 |
R-free | 0.29800 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.256 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.045 | |
Number of reflections | 28350 | |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 293 | 0.5% PEG4000(w/v), 0.01M sodium acetate, 10mg/ml protein in dark, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |