3G65
Crystal Structure of the Human Rad9-Rad1-Hus1 DNA Damage Checkpoint Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Detector technology | CCD |
Collection date | 2007-04-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.246, 70.673, 83.151 |
Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
Resolution | 82.000 - 2.900 |
R-factor | 0.2236 |
Rwork | 0.221 |
R-free | 0.27240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1axc |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.110 | 0.320 |
Number of reflections | 18454 | |
<I/σ(I)> | 3.1 | 2.2 |
Completeness [%] | 97.6 | 94 |
Redundancy | 4.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl, 17% PEG 2000-Mme, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl, 17% PEG 2000-Mme, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |