3G5Z
Antibodies Specifically Targeting a Locally Misfolded Region of Tumor Associated EGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.370, 94.800, 108.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.750 - 2.600 |
| R-factor | 0.2179 |
| Rwork | 0.214 |
| R-free | 0.30121 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.409 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.140 | 0.332 |
| Number of reflections | 9171 | |
| Completeness [%] | 98.4 | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 9 | 293 | 0.2M NH4 acetate, 24% PEG6K, 0.1M Bis-tris propane, pH9.0, EVAPORATION, temperature 293K |
