3G5C
Structural and biochemical studies on the ectodomain of human ADAM22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | H 3 |
Unit cell lengths | 122.090, 122.090, 208.690 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.360 |
Rwork | 0.245 |
R-free | 0.27270 |
Structure solution method | combined with SIRAS |
Starting model (for MR) | 1R54 AND 2ERO |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.490 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.069 | 0.389 |
Number of reflections | 49760 | |
<I/σ(I)> | 11.2 | 2.44 |
Completeness [%] | 96.6 | 91.5 |
Redundancy | 3.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |