3G3K
Crystal structure of the GluR6 ligand binding domain dimer I442H K494E K665R I749L Q753K E757Q mutant with glutamate and NaCl at 1.24 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.133, 113.844, 52.098 |
| Unit cell angles | 90.00, 115.22, 90.00 |
Refinement procedure
| Resolution | 35.898 - 1.240 |
| R-factor | 0.1469 |
| Rwork | 0.146 |
| R-free | 0.16500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.280 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.060 | 0.282 |
| Number of reflections | 151197 | |
| <I/σ(I)> | 18.5 | 3.39 |
| Completeness [%] | 98.6 | 86.5 |
| Redundancy | 4.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 17% PEG 4K, 12% ISOPROPANOL, 0.1M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
