3G3F
Crystal structure of the GluR6 ligand binding domain dimer with glutamate and NaCl at 1.38 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.185, 114.153, 52.364 |
Unit cell angles | 90.00, 115.31, 90.00 |
Refinement procedure
Resolution | 28.705 - 1.377 |
R-factor | 0.1533 |
Rwork | 0.152 |
R-free | 0.17490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s7y |
RMSD bond length | 0.012 |
RMSD bond angle | 1.179 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.430 |
High resolution limit [Å] | 1.377 | 1.380 |
Rmerge | 0.043 | 0.315 |
Number of reflections | 194151 | |
<I/σ(I)> | 14.4 | 2.41 |
Completeness [%] | 94.2 | 84 |
Redundancy | 1.5 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 16% PEG4K, 12% isopropanol, 0.1M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |