3G2U
VHS Domain of human GGA1 complexed with Sotilin C-terminal Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.8423 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.486, 72.728, 103.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.601 - 2.301 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jwf |
RMSD bond length | 0.009 |
RMSD bond angle | 0.986 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.500 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.081 | 0.349 |
Number of reflections | 16683 | |
<I/σ(I)> | 15.3 | 4.4 |
Completeness [%] | 99.0 | 99.6 |
Redundancy | 5.66 | 5.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 15%(w/v) PEG 5000 mmE, 0.2M NH4I, 0.3M 1,6-hexanediol, 0.1M MES-NaOH, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |