3G2G
S437Y Mutant of human muscle pyruvate kinase, isoform M2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-29 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.546, 137.885, 155.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.957 - 2.000 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zjh |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 22.000 | 22.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.092 | 0.029 | 0.999 |
| Number of reflections | 153815 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 99.7 | 100 | 99.1 |
| Redundancy | 7.2 | 7.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 291 | 25% PEG-1500, 0.2M ammonium sulfate, 0.1M sodium cacodylate, pH 5.5, vapor diffusion, temperature 291K |
