3G2G

S437Y Mutant of human muscle pyruvate kinase, isoform M2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-01-29
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	105.546, 137.885, 155.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.957 - 2.000
R-factor	0.227
Rwork	0.227
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zjh
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.000	22.000	2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmerge	0.092	0.029	0.999
Number of reflections	153815
<I/σ(I)>	10.1
Completeness [%]	99.7	100	99.1
Redundancy	7.2	7.3	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	291	25% PEG-1500, 0.2M ammonium sulfate, 0.1M sodium cacodylate, pH 5.5, vapor diffusion, temperature 291K