3FYK
Crystal structure of a benzthiophene lead bound to MAPKAP Kinase-2 (MK-2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-02 |
Detector | ADSC |
Wavelength(s) | 1.00 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 254.598, 254.598, 254.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.620 |
High resolution limit [Å] | 3.500 | 7.500 | 3.500 |
Rmerge | 0.083 | 0.038 | 0.352 |
Number of reflections | 8772 | ||
<I/σ(I)> | 19.778 | ||
Completeness [%] | 93.2 | 88.6 | 95.1 |
Redundancy | 7.5 | 7.4 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | MK-2 protein at 5 mg/ml equilibrated against a well solution of 1.6 - 2.0 M Sodium malonate at pH 5.4, vapor diffusion, hanging drop, temperature 298K |