3FYC

Crystal Structure of Dim1 from the thermophilic archeon, Methanocaldococcus jannaschi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-04-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	40.384, 72.274, 95.883
Unit cell angles	90.00, 91.87, 90.00

Refinement procedure

Resolution	32.000 - 2.150
R-factor	0.20946
Rwork	0.202
R-free	0.27332
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	composite model consisting of monomers from pdb entries 1QYR 2H1R and 1ZQ9
RMSD bond length	0.007
RMSD bond angle	1.123
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.770	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.091	0.361
Number of reflections	28619
<I/σ(I)>	12.4	4.7
Completeness [%]	94.9	91.9
Redundancy	6.62	6.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG 3350 (18%), 25 mM MES, 50 mM Na2HPO4, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K