3FY7
Crystal structure of homo sapiens CLIC3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-05 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 |
Unit cell lengths | 48.289, 48.673, 60.154 |
Unit cell angles | 69.11, 80.97, 74.80 |
Refinement procedure
Resolution | 28.030 - 1.950 |
R-factor | 0.234 |
Rwork | 0.231 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ahe |
RMSD bond length | 0.008 |
RMSD bond angle | 1.051 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.300 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.086 | 0.464 |
Number of reflections | 33742 | |
<I/σ(I)> | 8.6 | 1.9 |
Completeness [%] | 94.0 | 86.6 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | SOLUTION (15 MG/ML PROTEIN IN 20 MM HEPES*NAOH, PH 7.5, 200MM NACL) PLUS 3UL OF RESERVOIR SOLUTION (1.05M NH4SO4,0.225M LISO4, 0.1M TRIS-HCL PH 8.5), DROP EQUILIBRATED AGAINST 1 ML RESERVOIR SOLUTION , VAPOR DIFFUSION, HANGING DROP, temperature 293K |