3FY2
Human EphA3 Kinase and Juxtamembrane Region Bound to Substrate KQWDNYEFIW
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-11-02 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.824, 38.261, 76.365 |
Unit cell angles | 90.00, 102.05, 90.00 |
Refinement procedure
Resolution | 26.730 - 1.800 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fxx |
RMSD bond length | 0.011 |
RMSD bond angle | 1.259 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.040 | 34.040 | 1.880 |
High resolution limit [Å] | 1.780 | 5.630 | 1.780 |
Rmerge | 0.049 | 0.035 | 0.224 |
Total number of observations | 3476 | 14744 | |
Number of reflections | 29456 | ||
<I/σ(I)> | 7.855 | 14.1 | 3.3 |
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 3.6 | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20 mg/mL Protein, 25% PEG 3350, 0.04M Ammonium sulfate, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |