3FXY
Acidic Mammalian Chinase, Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-06-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.941, 92.360, 111.669 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.19868 |
| Rwork | 0.197 |
| R-free | 0.22838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.127 |
| Number of reflections | 91479 |
| <I/σ(I)> | 9.77 |
| Completeness [%] | 97.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 15% PEG 3350, 120 mM ammonium sulfate, 60 mM sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
