3FWO

The large ribosomal subunit from Deinococcus radiodurans complexed with Methymycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.873
Spacegroup name	I 2 2 2
Unit cell lengths	172.499, 415.691, 701.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.710
R-factor	0.278
Rwork	0.278
R-free	0.33700
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.700
Number of reflections	251032
<I/σ(I)>	6.4
Completeness [%]	94.7
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	291	HEPES, NH4CL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.8	291	HEPES, NH4CL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K