3FUX
T. thermophilus 16S rRNA A1518 and A1519 methyltransferase (KsgA) in complex with 5'-methylthioadenosine in space group P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.056, 79.908, 186.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.680 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.23900 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.494 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.740 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.080 | 0.380 |
Number of reflections | 91701 | |
<I/σ(I)> | 19.2 | 2.03 |
Completeness [%] | 94.8 | 95.4 |
Redundancy | 4.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch technique under oil | 8.5 | 277 | 20% v/v polyethylene glycol 300, 100 mM TRIS-HCl (pH 8.5), 5% w/v polyethylene glycol 8000, 10% v/v anhydrous glycerol, microbatch technique under oil, temperature 277K |