3FS4
Crystal structure determination of Ostrich hemoglobin at 2.2 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.794, 81.573, 101.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.620 - 2.220 |
| R-factor | 0.21765 |
| Rwork | 0.216 |
| R-free | 0.25602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1faw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.575 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.300 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 33356 | |
| <I/σ(I)> | 6.5 | 1.7 |
| Redundancy | 3.91 | 3.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 20% PEG 3350, 50mM Na Phosphate buffer, pH 7.20, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
