Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1992-01 |
Detector | FUJI |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 126.500, 191.700, 149.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.27600 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | CCP4 |
Refinement software | CNS (DEVELOPMENTAL) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.072 |
Total number of observations | 436000 * |
Number of reflections | 83821 |
Completeness [%] | 86.0 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 22 * | PH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | PIPES | 20 (mM) | |
3 | 1 | drop | 0.02 (%) | ||
4 | 1 | reservoir | PEG3000 | 15 (%) | |
5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 100 (mM) |