3FO9
Crystal structure of aldolase antibody 33F12 Fab' in complex with hapten 1,3-diketone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.830, 81.038, 174.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.560 - 1.900 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1axt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.308 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.560 | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 4.680 | 1.900 |
| Rmerge | 0.054 | 0.040 | 0.486 |
| Number of reflections | 71502 | ||
| <I/σ(I)> | 36.891 | ||
| Completeness [%] | 96.1 | 97.5 | 71.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.4 | 295 | 30% PEG 4000, 10% isopropanol, 0.1M Hepes., pH 7.4, EVAPORATION, temperature 295K |
