3FN1

E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 64 2 2
格子定数 [Å]	81.171, 81.171, 212.755
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	24.880 - 2.500
R因子	0.22599
Rwork	0.224
R-free	0.26501
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y8x
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	25.000	2.590
分解能 [Å] (高)	2.500	2.500
Rmerge_l_obs	0.057	0.599
独立反射数	13512
<I/σ(I)>	30.13	2.4
完全性 [%]	96.5	99.7
冗長性	4.7	4.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	9	291	0.7-0.8 M Na Citrate, 0.2 M NaC1, 0.1 M Bicine, 5 mM DTT, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 291K