3FMP
Crystal structure of the nucleoporin Nup214 in complex with the DEAD-box helicase Ddx19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.369, 112.915, 142.573 |
Unit cell angles | 90.00, 89.86, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.190 |
R-factor | 0.25114 |
Rwork | 0.249 |
R-free | 0.28317 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fmo |
RMSD bond length | 0.013 |
RMSD bond angle | 1.471 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 29717 | |
<I/σ(I)> | 14.3 | 3.6 |
Completeness [%] | 91.3 | 70.9 |
Redundancy | 6.8 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | PEG 3000, CHES buffer, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |