3FLO
Crystal structure of the carboxyl-terminal domain of yeast DNA polymerase alpha in complex with its B subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-16 |
Detector | ADSC Q315R |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.411, 142.633, 175.255 |
Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
Resolution | 31.900 - 2.500 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.21800 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.545 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.900 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.102 | 0.652 |
Number of reflections | 141597 | |
<I/σ(I)> | 5.2 | 1.2 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 7.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1M MES, 1.26M Ammonium Sulfate, 3mM TCEP, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |