3FKY
Crystal structure of the glutamine synthetase Gln1deltaN18 from the yeast Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 128.650, 129.940, 135.610 |
| Unit cell angles | 93.46, 104.61, 104.01 |
Refinement procedure
| Resolution | 65.218 - 2.950 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ojw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.250 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.106 | 0.407 |
| Number of reflections | 163752 | |
| <I/σ(I)> | 7.9 | 2.1 |
| Completeness [%] | 95.1 | 92.9 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.2M sodium acetate, 0.1M sodium citrate, 8% polyethylene glycol 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
