3FK2

Crystal structure of the RhoGAP domain of human glucocorticoid receptor DNA-binding factor 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-28
Detector	ADSC Q315
Wavelength(s)	0.97883
Spacegroup name	P 21 21 2
Unit cell lengths	165.025, 72.408, 72.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.431 - 2.800
R-factor	0.226
Rwork	0.223
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2osa
RMSD bond length	0.006
RMSD bond angle	1.005
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.157	0.057	0.598
Number of reflections	21961
<I/σ(I)>	5
Completeness [%]	98.1	99	89
Redundancy	5.1	5.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	26% PEG 3350, 0.1M Bis-Tris, 0.2M lithium sulfate, 0.001M DTT, 1:100 (w/w) trypsin, pH 6.0, vapor diffusion, sitting drop, temperature 291K